Analysis of the nucleic acid detection quality assessment results from novel coronavirus laboratories in 43 disease control institutions in Qinghai province in 2021

Objective To assess the quality of nucleic acid testing in 43 Novel Coronavirus laboratories of disease control institutions in Qinghai Province that have passed the acceptance inspection, so as to ensure the accuracy and reliability of nucleic acid testing results. Methods Five samples for quality control assessment were distributed to each testing institution. The quality of nucleic acid testing was carried out following the requirements of the testing technical guidelines in the Novel Coronavirus Pneumonia Prevention and Control Program (Seventh Edition) issued by the Chinese Center for Disease Control and Prevention. Each detection reagentwas prepared by the member units participating in the assessment. The nucleic acid detection ability of each institution was evaluated by comparing the testing results with the expected results of the assessment samples. Results Fortythree disease control institutions participated in the assessment, all the testing results were collected within the specified time. The overall compliance rate of this assessment was 100. 00%. A total of 10 manufacturers' nucleic acid extraction kits and 9 manufacturers' nucleic acid amplification kits were involved. One hundred percent of the kits detected the ORF1ab gene and N gene of the Novel Coronavirus. Conclusion The nucleic acid detection capacity of the disease control institutions in Qinghai has been further improved by making this Novel Coronavirus quality control assessment. The assessment createsa solid foundation for the prevention and control of the Novel Coronavirus epidemic in Qinghai. © 2023, Editorial Department of Medical Pest Control. All rights reserved.

Analysis of the nucleic acid detection quality assessment results from novel coronavirus laboratories in 43 disease control institutions in Qinghai province in 2021

Objective To assess the quality of nucleic acid testing in 43 Novel Coronavirus laboratories of disease control institutions in Qinghai Province that have passed the acceptance inspection, so as to ensure the accuracy and reliability of nucleic acid testing results. Methods Five samples for quality control assessment were distributed to each testing institution. The quality of nucleic acid testing was carried out following the requirements of the testing technical guidelines in the Novel Coronavirus Pneumonia Prevention and Control Program (Seventh Edition) issued by the Chinese Center for Disease Control and Prevention. Each detection reagentwas prepared by the member units participating in the assessment. The nucleic acid detection ability of each institution was evaluated by comparing the testing results with the expected results of the assessment samples. Results Fortythree disease control institutions participated in the assessment, all the testing results were collected within the specified time. The overall compliance rate of this assessment was 100. 00%. A total of 10 manufacturers' nucleic acid extraction kits and 9 manufacturers' nucleic acid amplification kits were involved. One hundred percent of the kits detected the ORF1ab gene and N gene of the Novel Coronavirus. Conclusion The nucleic acid detection capacity of the disease control institutions in Qinghai has been further improved by making this Novel Coronavirus quality control assessment. The assessment createsa solid foundation for the prevention and control of the Novel Coronavirus epidemic in Qinghai. © 2023, Editorial Department of Medical Pest Control. All rights reserved.

Mediating role of psychological resilience between social support and negative emotions in nursing interns in the post-COVID-19 era: A cross-sectional survey

Background: The increasing burden of mental health problems continues in the post-COVID-19 era, and nursing interns were particularly likely to experience negative emotions during the pandemic. Both psychological resilience and social support affect negative emotion, but the relationship among the three has not been explored in nursing interns in the postpandemic era. Objectives: To explore the current prevalence of negative emotions among nursing interns and the role of psychological resilience in mediating the relationship between social support and negative emotions in the postpandemic era. Methods: A cross-sectional survey of 788 nursing interns was conducted. The instruments included Psychological Resilience Scale, Social Support Scale, Beck Anxiety Scale and Beck Depression Scale. Structural equation modeling was applied to analyze the mediating role of psychological resilience. Results: The prevalence of anxiety disorder among nursing interns was 24.7%, while that of depression was 10.5%. Pearson correlation analysis showed that both social support and psychological resilience negatively correlated with negative emotions, while psychological resilience positively correlated with social support. Psychological resilience showed a partial mediating effect (53.9%) between social support and negative emotion, with an effect value of −0.1456. Conclusion: Psychological resilience and social support protect nursing students from negative emotions, and psychological resilience partially mediates the relationship between social support and negative emotion in the postpandemic era. © 2023 Wiley Periodicals LLC.

[Bedside monitoring of lung perfusion by electrical impedance tomography].

As a non-invasive and radiation-free bedside imaging method, electrical impedance tomography (EIT) can perform real-time regional pulmonary ventilation evaluation and pulmonary blood flow monitoring for patients, thus realizing bedside ventilation/perfusion matching visualization, effectively guiding the pathophysiological mechanism of hypoxemia, and providing a new method for the study of pulmonary blood flow. EIT has also played a unique and irreplaceable role in COVID-19 research and treatment. At the same time, as functional imaging, the operation details and image reconstruction algorithm of this technology still need to be further optimized by more researches to provide a more robust evaluation in clinical application. In this paper, EIT pulmonary blood flow monitoring methods, operation and implementation of monitoring indicators, application and related research progress will be described.

The impact of regional port integration in the context of the epidemic

The spread of the COVID-19 epidemic in 2020 has extremely serious negative impacts on the global freight supply chain with regional port groups as its core. In order to reconstruct the global freight supply chain as soon as possible, alleviate the vicious competition of regional port groups and promote the development of regional integration, the advantages of port integration are increasingly prominent. To this end, based on Gounod's game theory, a three-stage game model of port integration is constructed with the overall objective of obtaining the maximum economic benefits for the regional port group, based on the current characteristics of the global freight supply chain impacted by the COVID-19 epidemic, and empirical analysis is conducted using the data of the Liaoning port group, and the reverse induction method is used to quantitatively analyze the specific impact of regional port integration on the economic benefits of the port and determine the performance of the regional port group under the background of the epidemic The theoretical basis is laid for the rational decision of port managers. The calculation results show that there is a threshold effect in the integration process of the Liaoning regional port group, and the gradual deepening of the integration of the Liaoning regional port group can effectively mitigate the negative impact of the COVID-19 epidemic on the regional port group. © 2021 SPIE

FROM EMERGING TO MATURE MARKETS : OPTIMUM SALES STRATEGIES IN THE CRUISE INDUSTRY

In service industries, such as cruise tourism, intermediaries (agents) are crucial in the initial stages of foreign market penetration and business development. This is particularly important in emerging and protected markets, such this of China a few years back. As the market matures, however, and the consumer becomes savvier, the role of the agent declines, and direct sales become more popular. We combine game theory and discrete consumer choice theory to construct a decision-making tool for cruise companies, aiming to assist them in choosing the right pricing and sales strategy under different market circumstances and shocks, such as the COVID-19 pandemic. Our numerical experiments convincingly demonstrate that collaborative pricing and sales is a winning strategy in most market environments, including unforeseen situations of major shocks. Competitive pricing and sales, instead, reduces profitability for both company and agent. Single channel sales strategies, i.e., either direct sales or through the agent, appear to be the less performing options and, were this to be the case, the cruise company would be better off by selling only to travel agents. Although our methodology and results are of generic value and easily generalizable, China - used here as an illustrative example only - has nonetheless proven to be an excellent case study of the way cruise product pricing and sales strategies have evolved, in an effort to cope with the transition from an emerging - to a mature cruise market.

Design of a Personalized Recommendation System for Learning Resources based on Collaborative Filtering

At present, personalized recommendation system has become an indispensable technology in the fields of e-commerce, social network and news recommendation. However, the development of personalized recommendation system in the field of education and teaching is relatively slow with lack of corresponding application.In the era of Internet Plus, many colleges have adopted online learning platforms amidst the coronavirus (COVID-19) epidemic. Overwhelmed with online learning tasks, many college students are overload by learning resources and unable to keep orientation in learning. It is difficult for them to access interested learning resources accurately and efficiently. Therefore, the personalized recommendation of learning resources has become a research hotspot. This paper focuses on how to develop an effective personalized recommendation system for teaching resources and improve the accuracy of recommendation. Based on the data on learning behaviors of the online learning platform of our university, the authors explored the classic cold start problem of the popular collaborative filtering algorithm, and improved the algorithm based on the data features of the platform. Specifically, the data on learning behaviors were extracted and screened by knowledge graph. The screened data were combined with the collaborative filtering algorithm to recommend learning resources. Experimental results show that the improved algorithm effectively solved the loss of orientation in learning, and the similarity and accuracy of recommended learning resources surpassed 90%. Our algorithm can fully satisfy the personalized needs of students, and provide a reference solution to the personalized education service of intelligent online learning platforms. © 2022, North Atlantic University Union NAUN. All rights reserved.

[Countermeasures and treatment for aortic acute syndrome with 2019 coronavirus disease].

The 2019 coronavirus disease(COVID-19) has cost a great loss to the health and economic property of Chines people. Under such a special circumstance, how to deal with such patients with acute aortic syndrome has become a serious challenge. Rapid diagnosis of concomitant COVID-19, safe and effective transportation, implementation of the interventional procedure, protection of vascular surgical team and postoperative management and follow-up of such patients have become urgent problems for us. Combined with the latest novel government documents, the literature and the experiences from Wuhan, we answered the above questions briefly and plainly. We also hope to inspire the national vascular surgeons to manage critical emergencies in vascular surgery and even routine vascular diseases with COVID-19, as a final point to limit the severe epidemic situation, and minimize the damage of COVID-19.

[Standardized diagnosis and treatment of colorectal cancer during the outbreak of corona virus disease 2019 in Renji hospital].

Corona virus disease 2019 (COVID-19) is currently raging in China. It has been proven that COVID-19 can be transmitted from human to human and cause hospital infection, which seriously threatens surgical staffs and inpatients. Although colorectal surgery is not a front-line subject in the fight against the epidemic, but in this special situation, it is a difficult task to provide the highest quality medical services and ensure the orderly clinical work, on the premise of maximizing the protection for patients and their families, health of medical staff, and the safety of wards and hospitals, We summarize how to carry out the clinical practice of colorectal surgery under the situation of the prevention and control of the COVID-19 epidemiology, including the procedures of diagnose and treatment for emergency patients with colorectal tumor, and share the experiences of the diagnosis of colorectal tumor, the management of patients with colorectal cancer who are scheduled to be admitted for surgery, the protection of wards, the perioperative management. More importantly, we introduce in detail the operative management and perioperative management of colorectal surgery patients suspected or diagnosed with new coronary pneumonia, including prevention and control measures for medical staff, operating rooms and surgical instruments. The main points are as follows: (1) Multidisciplinary team (MDT) must be run through the diagnosis and treatment of colorectal cancer. The members include not only routine departments, but also respiratory department and infectious department. (2) Colonoscopy examination may cause cross infection of COVID-19 to patients and doctors. Therefore, it is prior to examine the emergency cases and life-threatening patients (bleeding, obstruction, gastrointestinal foreign bodies, etc.). If the emergent patients (intestinal obstruction) with suspected or confirmed COVID-19, the surgeons must perform emergency surgery, and intestinal decompressive tube through colonoscopy is not recommended. (3) The colorectal cancer patients with suspected or confirmed COVID-19 should be placed in the isolated room with separate medical devices, and the operative room with negative pressure (under -5 Pa) must be separated. All disposable medical items, body fluids and feces of the patients in perioperative periods must be unified disposed according to the medical waste standard. (4) The surgical medical workers who process colorectal cancer patients with COVID-19 must be protected by three-level. After operation, the medical workers must receive medical observation and be isolated for 14 days. We hope our "Renji experience" will be beneficial to colleagues.

Correlation between travellers departing from Wuhan before the Spring Festival and subsequent spread of COVID-19 to all provinces in China ;Journal of Travel Medicine ;Oxford Academic

Highlight We found a strong correlation between travel volumes departing from Wuhan, Hubei Province before the Spring Festival and the extent of amplification of the outbreak of COVID-19 in China in 2020, with 100 top cities. Almost 70% of exportations were within cities in Hubei province.